今回は、神経科学の研究で広く使われているツール、試薬について、その使用に注意を求めている最近の論文を紹介します。
（１）AAV9は高力価で使うと毒性がある
Severe toxicity in nonhuman primates and piglets following high-dose intravenous administration of an AAV vector expressing human SMN
http://online.liebertpub.com/doi/abs/10.1089/hum.2018.015
https://www.the-scientist.com/?articles.view/articleNo/51526/title/Severe-Toxicity-Reported-in-High-Dose-AAV-Gene-Therapy-in-Animals/
Severe toxicity in nonhuman primates and piglets following high-dose intravenous administration of an AAV vector expressing human SMN
http://online.liebertpub.com/doi/abs/10.1089/hum.2018.015
https://www.the-scientist.com/?articles.view/articleNo/51526/title/Severe-Toxicity-Reported-in-High-Dose-AAV-Gene-Therapy-in-Animals/
（２）蛍光カルシウムイオン指示薬はNa, K-ATPaseを阻害して、細胞機能に影響を与える
Fluorescent Ca2+ indicators directly inhibit the Na,K-ATPase and disrupt cellular functions
（３）Cre-loxシステムを使う際の注意点
Detecting and Avoiding Problems When Using the Cre–lox System
今更感がありますが。。。
